Regular ArticleInvolvement of Gαq/11 in m3-Muscarinic Receptor Stimulation of Phosphatidylinositol 4,5 Bisphosphate-Specific Phospholipase C in Rat Parotid Gland Membranes
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Selectivity and evolutionary divergence of metabotropic glutamate receptors for endogenous ligands and G proteins coupled to phospholipase C or TRP channels
2014, Journal of Biological ChemistryCitation Excerpt :Among the four types of dominant negative mutants of Gαi/o proteins (i1, i2, i3, and oA), the dominant negative mutants of Gαi2 and Gαo blocked the l-glutamate mediated mGluR1 activation of TRPC4β the most, and mutant Gαi1 or Gαi3 blocked the response to a lesser extent (Fig. 7, C–F). To test for the possibility that the dominant negative isoforms at high levels simply block activation of Gq, we titrated cells with increasing amounts of dominant-negative-expressing plasmids (Fig. 7, C–F) to reveal that mGluR1 signaling to TRPC4β can be almost completely suppressed, whereas the same level of dominant negative subunits has little effect on signaling to TRPC4β by the M3 muscarinic acetylcholine receptor, a Class A GPCR known to work through activation of Gαq/11 (45, 46). In addition, the same levels of dominant-negative Gαo that completely suppress TRPC4β activation, have no effect on Ca2+ release induced by endogenous Gq/11 in the same cell line (Fig. 8, A–B).
Water channels and zymogen granules in salivary glands
2006, Journal of Pharmacological SciencesSpatial microenvironment defines Ca<sup>2+</sup> entry and Ca<sup>2+</sup> release in salivary gland cells
2005, Biochemical and Biophysical Research CommunicationsCitation Excerpt :α-Amylase was detected in the preparation from acini but not in that from ducts, whereas kallikrein was found only in ducts. Previously, acinar cells were reported to express the M3 muscarinic receptor [10,11], but the muscarinic receptor subtype in duct cells has been unknown. The muscarinic receptor subtypes were examined in the acinar and duct cells.
cAMP potentiates ATP-evoked calcium signaling in human parotid acinar cells
2004, Journal of Biological ChemistryEffect of SNI-2011 on amylase secretion from parotid tissue in rats and in neuronal nitric oxide synthase knockout mice
2003, European Journal of Pharmacology