A comparison of transfer RNA isoaccepting species between collagenous and noncollagenous tissues in the embryonic chick

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Abstract

Transfer RNA was isolated from different organs of 17-day-old chick embryos and the acceptor activity for each of the 20 amino acids was determined. The most abundant acceptor activities found in tRNA from tendon cells were for glycine, arginine, proline and alanine. When compared to the average acceptor activity found in brain, liver and heart, the tendon tRNA showed an increase in acceptor activity of 33% in glycine, 40% in arginine and 83% in proline. Reversed phase chromatography of the tRNA charged with glycine demonstrated that the increase in glycyl-tRNA in tendon could be accounted for by an increase in one of four major isoaccepting species. Such an increase in a single species was also observed in tRNA isolated from calvaria. The codon response of this species was shown to differ from that of the other glycyl-tRNA species. No major differences in the relative proportions of isoaccepting species could be demonstrated for any other amino acid. These results suggest that a characteristic complement of tRNA species may be associated with collagen synthesis.

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    This investigation was supported by the National Institutes of Health research grants No. AM-14439, AM-14526, and a National Institute of Dental Research U.S.P.H.S. grant, No. DE-02623. This investigation was submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy, Department of Microbiology, School of Medicine, University of Pennsylvania.

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