Developmental changes of the content of acetyl-CoA carboxylase mRNA in chicken liver

doi:10.1016/0003-9861(88)90263-9

Archives of Biochemistry and Biophysics

Volume 266, Issue 2, 1 November 1988, Pages 313-318

https://doi.org/10.1016/0003-9861(88)90263-9 Get rights and content

Abstract

Utilizing RNA blot hybridization and immunoblotting techniques, the changes of the hepatic contents of acetyl-CoA carboxylase mRNA and of the enzyme protein in growing chicks have been investigated. In the post-hatching period, the hepatic mRNA level markedly increased at least 70-fold when compared to that before hatching. This increase was not observed in chicks receiving no diet. These changes were closely paralleled with the rise of the hepatic content of acetyl-CoA carboxylase protein in chicks up to 10 days old. Neither the acetyl-CoA carboxylase mRNA level nor the enzyme quantity significantly changed in heart. It is concluded from these results that the developmental regulation of acetyl-CoA carboxylase in the post-hatching period of chicks is tissue specific and occurs primarily at a pretranslational step. The content of acetyl-CoA carboxylase mRNA in adult chicken liver was low, which is comparable to those in embryos at 3 days before hatching and chicks at hatching day. Although acetyl-CoA carboxylase mRNA was detected in adult chicken brain, heart, lung, kidney, uropygial gland, spleen, testis, and chest muscle as well as liver, the mRNA level in these tissues was much lower than that in liver of growing chicks.

References (36)

S. Numa et al.
P.W.F. Fischer et al.
Arch. Biochem. Biophys
(1978)
P.W. Majerus et al.
J. Biol. Chem
(1969)
T. Takai et al.
FEBS Lett
(1987)
T. Takai et al.
J. Biol. Chem
(1988)
A.P. Feinberg et al.
Anal. Biochem
(1983)
R. Hawkes et al.
Anal. Biochem
(1982)
O.H. Lowry et al.
J. Biol. Chem
(1951)
K.-H. Lee et al.
J. Biol. Chem
(1979)
K. Wada et al.
FEBS Lett
(1985)

A.G. Goodridge

J. Biol. Chem

(1973)

D.H. Bai et al.

J. Biol. Chem

(1986)

F. López-Casillas et al.

Arch. Biochem. Biophys

(1987)

A.G. Goodridge et al.

Arch. Biochem. Biophys

(1984)

S.M. Morris et al.

J. Biol. Chem

(1982)

D.W. Back et al.

J. Biol. Chem

(1986)

S.S. Chirala et al.

J. Biol. Chem

(1987)

S.J. Wakil et al.

Annu. Rev. Biockem

(1983)

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It has two isoforms that are encoded by two different genes (Abu-Elheiga et al., 1997). The one is accα, a cytosolic enzyme, whose product (malonyl-CoA) is the committed substrate in the biosynthesis of long-chain fatty acids (Takai et al., 1988). And the other is accβ, a mitochondrial enzyme, and its malonyl-CoA product regulates fatty acid oxidation by inhibiting the shuttle that transports long-chain acyl-CoAs from the cytosol to the mitochondria for oxidation (Abu-Elheiga et al., 1997).
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Fatty acid synthase (FAS) can catalyze de novo fatty acid synthesis (Cowey and Walton, 1989). Cytosolic enzyme acetyl-CoA carboxylase alpha (ACCα), its production of malonyl-CoA was the pivotal step in the biosynthesis of long-chain fatty acids, which also played a crucial part in biosynthesis of body fatty acids (Takai et al., 1988; Qian et al., 2015). Glucose 6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) were vital regulatory enzymes related with NADPH production, which was essential for fatty acid biosynthesis (Chen et al., 2013; Zheng et al., 2013).
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Furthermore, accα and fas are two genes that play important roles in biosynthesis of body fatty acid (Wakil, 1961). As a key enzyme, the accα's production of malonyl-CoA is a committed step in biosynthesis of long-chain fatty acids (Takai et al., 1988; Zheng et al., 2013), and the function of FAS is to catalyze de novo fatty acid synthesis (Zheng et al., 2013; Cowey and Walton, 1989). In this current experiment, the expression of accα and fas both decreased with increased dietary n-3 HUFA level, and these results were the same as the n-6 PUFAs (C18:2n-6, C18:3n-6, C20:2n-6 and total n-6-PUFA) contents in the liver and muscle.
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^☆: This work was supported by research grants from the Ministry of Health and Welfare, and the Ministry of Education, Science and Culture of Japan.

^☆☆: The Materials and Methods section and Figs. 1,2, and 4 of this paper appear as a Miniprint Supplement.

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Developmental changes of the content of acetyl-CoA carboxylase mRNA in chicken liver☆,☆☆

Abstract

Arch. Biochem. Biophys

J. Biol. Chem

FEBS Lett

J. Biol. Chem

Anal. Biochem

Anal. Biochem

J. Biol. Chem

J. Biol. Chem

FEBS Lett

J. Biol. Chem

J. Biol. Chem

Arch. Biochem. Biophys

Arch. Biochem. Biophys

J. Biol. Chem

J. Biol. Chem

J. Biol. Chem

Annu. Rev. Biockem