Antisense src Expression Inhibits U937 Human Leukemia Cell Proliferation in Conjunction with Reduction of c-myb Expression

doi:10.1006/bbrc.1994.1801

Biochemical and Biophysical Research Communications

Volume 201, Issue 2, 15 June 1994, Pages 1001-1007

https://doi.org/10.1006/bbrc.1994.1801 Get rights and content

Abstract

To elucidate the role of pp60^c-src in U937 human monoblastoid leukemia cell proliferation, recombinant plasmids containing the src gene or myb gene, which could produce antisense src or antisense myb RNA after dexamethasone treatment, were constructed and transfected into U937 cells (U937-ASRC, U937-AMYB). pp60^c-src synthesis in U937-ASRC was diminished by the third day after induction of antisense src RNA and the eel proliferation was reduced, furthermore, the amount of p75^c-myb was significantly decreased by the third day. p75^c-myb synthesis in U937-AMYB was diminished by the second day after induction of antisense myb RNA and the cell growth was significantly inhibited but the amount of pp60^c-src in U937-AMYB was not reduced. These results suggest that a decrease in the amount of pp60^c-src leads to an inhibition of p75^c-myb expression and subsequent reduction in the U937 cell proliferation.

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Cited by (14)

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Regular ArticleAntisense src Expression Inhibits U937 Human Leukemia Cell Proliferation in Conjunction with Reduction of c-myb Expression

Abstract

Regular Article
Antisense src Expression Inhibits U937 Human Leukemia Cell Proliferation in Conjunction with Reduction of c-myb Expression