The glucose polymer of PC12 cells is susceptible to trypsinization

https://doi.org/10.1016/0006-291X(89)92395-4Get rights and content

Abstract

The glucose polymer of PC12 cells (Rasilo, M.-L. and Yamagata, T., 1988, FEBS Letters 227, 191–194 and Rasilo, M.-L. and Yamagata, T., 1988, Journal of Biochemistry, 104, 742–754) was found to be located on the cell surface. The polymer was liberated from the galactose-labeled cells with a trypsin treatment: maximally 65% of the glucose polymer was liberated, compared with 36% of the large glycopeptides. Even when the cells were incubated with the saline about one fourth of the polymer moved into the solution, but less than 8% of the large glycopeptides. Phosphatidyl inositol-specific phospholipase C failed to liberate the polymer.

References (19)

  • L.A. Greene et al.
  • R.K. Margolis et al.

    J. Biol. Chem

    (1983)
  • G.A. Schwarting et al.

    Neuroscience

    (1986)
  • M. Shimamura et al.

    J. Biol. Chem

    (1988)
  • M.-L. Rasilo et al.

    FEBS Letters

    (1988)
  • M. Laskowski
  • J.C. McGuire et al.

    Cell

    (1978)
  • H. Ikezawa et al.
  • L.A. Greene et al.
There are more references available in the full text version of this article.

Cited by (2)

Visiting Guest Researcher, Present address: Department of General Chemistry, Faculty of Agriculture and Forestry, University of Helsinki, Viikki, 00710 Helsinki, Finland.

View full text