An NMR study of the oxidation state of Cu,Zn superoxide dismutase in human red blood cells

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Abstract

The oxidation state of Cu,Zn superoxide dismutase was investigated by 19F-NMR spectroscopy in intact red blood cells and in their lysates. The superoxide dismutase concentration was determined in the red cells both by activity and by F nuclear relaxation rate measurements and the results obtained showed that the high relaxation rate of F in erythrocytes is mainly due to the presence of superoxide dismutase. The relaxation rate of F was unaffected or slightly increased by the addition of a superoxide ion generating system to the cells or to their lysates so indicating that superoxide dismutase is fundamentally in steady state. The results are discussed in terms of the possible reactions of the enzyme in erythrocytes.

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