Isolation and identification of hemin as an endogenous Na+K+-ATPase inhibitor from porcine blood cells

https://doi.org/10.1016/0006-291X(91)91784-AGet rights and content

Abstract

A substance which is a potent inhibitor of Na+K+-ATPase activity and competitively displaces [3H]ouabain binding to this enzyme was isolated from porcine blood cells. From its chemical and physicochemical properties, this activity was identified as hemin (chloroprotohemin IX). Hemin showed a dose dependent curve for Na+K+-ATPase inhibitory activity similar to that of ouabain and displaced [3H] ouabain binding as potent as 1100 of ouabain itself.

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