In vivo expression of rat liver c-erbA β thyroid hormone receptor in yeast (Saccharomyces cerevisiae)

https://doi.org/10.1016/0006-291X(90)91367-2Get rights and content

Abstract

To study thyroid hormone receptor (TR), we developed an in vivo expression system in yeast by using a copper-responsive yeast metallothionein promoter and ubiquitinfusion protein technology. The cDNA encoding full-length rat liver TRβ was expressed under the control of copper. The [125I]T3 binding activities to yeast extracts were significantly correlated with the added copper sulfate into the medium. Partially purified TR from the transformed yeast had a high hormone binding affinity (Kd=0.34) for T3 and could bind thyroid hormone response element in gel retardation analysis.

References (23)

  • T.R. Butt et al.

    J. Biol. Chem

    (1988)
  • P. Mak et al.

    J. Biol. Chem

    (1989)
  • M.B. Murray et al.

    J. Biol. Chem

    (1988)
  • O.H. Lowry et al.

    J. Biol. Chem

    (1951)
  • A. Inoue et al.

    Anal Biochem

    (1983)
  • C.K. Glass et al.

    Cell

    (1988)
  • J.W. Apriletti et al.

    J. Biol. Chem

    (1988)
  • K.H. Lin et al.

    J. Biol. Chem

    (1990)
  • T.N. Lavin et al.

    J. Biol. Chem

    (1988)
  • M.F. Norman et al.

    J. Biol. Chem

    (1989)
  • J.H. Oppenheimer et al.
  • Cited by (0)

    View full text