Phospholipid activation of Lactobacillusplantarum undecaprenyl pyrophosphate synthetase

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Abstract

The ability of a number of phospholipids to stimulate Lactobacillusplantarum undecaprenyl pyrophosphate synthetase was investigated. The detergent Triton X-100, which is added to stabilize the enzyme during purification and is required for invitro activity, was removed with the non-ionic resin XAD-2. The effects of cardiolipin, phosphatidyl ethanolamine, phosphatidyl choline, and phosphatidyl glycerol on the activity of XAD-2 treated undecaprenyl pyrophosphate synthetase were determined. Of the phospholipids studied only cardiolipin stimulated invitro enzymic activity as effectively as Triton X-100.

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This work was supported by a grant from the National Science Foundation (GB-34246).

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Present address: Dept. of Chemistry, University of Wisconsin River Falls, River Falls, Wisconsin 54022.

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