Elsevier

Analytical Biochemistry

Volume 134, Issue 2, 15 October 1983, Pages 398-405
Analytical Biochemistry

Chromatography of permethylated oligosaccharide alditols

https://doi.org/10.1016/0003-2697(83)90315-9Get rights and content

Abstract

A new chromatographic method which enables the separation of permethylated oligosaccharide alditols has been developed. The method entails chromatography on precoated silica gel plates using benzene-methanol (16:1, vv or 10:1 vv) as developing solvent. Separations of disaccharides were obtained on the basis of glycosidic linkage and anomeric configuration; the method can accomodate oligosaccharides containing up to 15 glycose units. The combined use of thin-layer chromatography and gas-liquid chromatography provides a powerful approach for the characterization of oligosaccharides. Retention indices are given of permethylated oligosaccharide alditols on a fused-silica capillary column bonded with DB-1.

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    This work was supported by NIH Grant AM12434.

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