Elsevier

Analytical Biochemistry

Volume 12, Issue 3, September 1965, Pages 497-508
Analytical Biochemistry

A critical study of amperometric titration methods for sulfhydryl determination in native and denatured bovine serum albumin

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Abstract

By amperometric titration with mercuric chloride or ethylmercuric or methylmercuric chloride in tris or ammonia buffers the sulfhydryl content of crystalline bovine serum albumin is found the same in the native state as in 8 M urea or 1% sodium dodecyl sulfate (SDDS). Amperometric titrations with silver nitrate yield high results in 8 M urea and 1% SDDS, both at pH 7 to 7.4. The rotated mercury wire electrode is found very suitable for amperometric titrations with mercuric chloride or ethyl- (methyl-) mercuric chloride.

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This investigation was supported by Public Health Service Research Grants No. CA-00721-15 and CA-00721-16 from the National Cancer Institute.

2

Present address: Concordia College, Moorhead, Minn. 56560.

3

Present address: 10-66, Hommachi, Toyonaka-city, Osaka, Japan.

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