Elsevier

Analytical Biochemistry

Volume 86, Issue 2, 1 June 1978, Pages 519-525
Analytical Biochemistry

Enzymatic synthesis of [U-14C]ribose-labeled inosine

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Abstract

A convenient enzymatic method to synthesize inosine specifically labeled in the ribose moiety is presented. The synthesis is effected in two stages subjecting [U-14C]inosine to the action of: (1) purine-nucleoside phosphorylase, leading to the formation of [U-14C]ribose 1-phosphate, and then (2) adenosine phosphorylase and adenosine deaminase plus unlabeled adenine, which permits the reformation of inosine containing the labeled ribose formed in the first stage.

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