Elsevier

Analytical Biochemistry

Volume 91, Issue 1, November 1978, Pages 367-369
Analytical Biochemistry

Short communication
A simple procedure for regeneration of an organomercurial agarose column

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Abstract

Organomercurial agarose has been used in the purification of various thiol compounds including enzymes (1). Thiol compounds are first adsorbed on a column of organomercurial agarose, and then eluted with a second thiol compound, e.g., 2-mercaptoethanol (2-ME)1 and cysteine. Although this column can be used repeatedly, a usual method for regeneration of the column is to remove the second thiol by HgCl2. It would be desirable to regenerate the column without using HgCl2, since it is biohazardous. In the study of the purification of a thiol-containing enzyme, we found that organomercurial agarose, which had previously been treated with 2-ME, could adsorb the enzyme and that the enzyme was eluted with 2-ME. This finding led us to examine whether the column can be used repeatedly without the regeneration using HgCl2.

References (4)

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    J. Biol. Chem

    (1969)
There are more references available in the full text version of this article.

Cited by (3)

1

Abbreviations used: 2-ME, 2-mercaptoethanol; MAA, p-chloromercurianiline-agarose.

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