A novel spectroscopic titration method for determining the dissociation constant and stoichiometry of protein-ligand complex☆
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2022, Biochemical and Biophysical Research CommunicationsCitation Excerpt :Protein solution (1.0 μM, 2 mL) prepared in HEPES buffer (10 mM, pH 7.4) was disposed by a continuous injection of Merbromin, with the final ratios of Merbromin to protein from 1 to 12. Data were analyzed according to the previous report [16]. Molecular docking studies of the binding between Merbromin and 3CLpro were performed by using the Molecular Operating Environment (MOE) software.
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2021, European Journal of Medicinal ChemistryCitation Excerpt :Furthermore, to be a control, PBS buffer with isometric DMSO is introduced to duplicate the whole process as ligand, to exclude the nonspecific quenching. Data fitting was executed on OriginPro 2016 (Origin, Inc), and the one site binding analysis for spectrum titration experiment according to literature was employed here to determine the dissociation constant (KD) [44]. 3T3-L1 cell seeded at 24-well plate were cultured for 2 days after confluence.
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Journal Article 2041 of the North Dakota Agricultural Experiment Station.