Elsevier

Analytical Biochemistry

Volume 69, Issue 2, December 1975, Pages 607-617
Analytical Biochemistry

A new principle for activity measurement of ADP or ATP dependent enzymes: Fluorescence quenching of ϵ-ADP and ϵ-ATP by divalent metal ions

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Abstract

The divalent metal ions Cu2+, Co2+, Mn2+, and Zn2+ form complexes with the fluorescent etheno analogs of the adenine nucleotides. The fluorescence intensity is thereby diminished. The binding strength of the metals to etheno-adenosine triphosphate is higher than to etheno-adenosine di- and monophosphate. The quenching effect of the divalent metal ions can be exploited as a simple routine activity measurement for various kinases and phosphohydrolases.

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