Determination of glutathione in biological material by flow-injection analysis using an enzymatic recycling reaction
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Drug-induced oxidative stress as a mechanism of toxicity
2023, Essentials of Pharmatoxicology in Drug Research: Toxicity and Toxicodynamics: Volume 1A novel colorimetric and fluorometric probe for biothiols based on MnO<inf>2</inf> NFs-Rhodamine B system
2020, Analytica Chimica ActaCitation Excerpt :Consequently, sensitive detection of biothiols has become an urgent demand for the protection of human health. Up to date, various analytical methods have been established for biothiols detection, including high-performance liquid chromatography (HPLC), electrochemistry, capillary electrophoresis and flow injection analysis [22–25]. Although accurate and reliable results can be obtained, these methods are high-cost, and depend on sophisticated instruments and time-consuming sample pre-treatments [26].
Activated charcoal significantly improved the reliability of methods for quantitative analysis of endogenous substances in biological specimens: Glutathione and cysteine as cases
2018, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life SciencesCitation Excerpt :Thus, the simultaneous quantification of GSH and CYS would be useful to interpret more precisely the role of GSH whether as an indicator of diseases or as a therapeutic drug. Several previous analytical methods, including gas chromatography, capillary electrophoresis, high performance liquid chromatography (HPLC), LC-MS, LC-MS/MS, and a novel benzothiazole-based fluorescent probe, have been used to quantify GSH and other thiol compounds in biological matrices [18–29]. A decade years ago, HPLC with ultraviolet (UV) and fluorescence (FL) detectors is widely used because of its convenience, high reproducibility and low cost [22–24].
Glutathione
2015, Profiles of Drug Substances, Excipients and Related MethodologyCitation Excerpt :Some aspects of the mechanism of the reaction are discussed. Redegeld et al. [73] presented a sensitive and specific assay method for glutathione using recycling reaction followed by spectrophotometric detection in a flow-injection analysis system. The method provides specific amplification of the response to glutathione by combined use of the enzyme glutathione disulfide reductase and the chromogenic reagent 5,5′-dithiobis(2-nitrobenzoic acid).
Determination of reduced glutathione, cystein and total thiols in pine pollen powder by in situ derivatization
2014, Microchemical JournalCitation Excerpt :Most analytical methods for thiol compounds in biological samples found in the literature generally utilize a thiol selective derivatizing agent, which can react with thiol compounds to form either fluorescent or non-fluorescent derivatized products. Among these methods, 5,5′-dithio-bis(2-nitrobenzoic acid) (DTNB) and o-phthaldehyde (OPA) are by far the most commonly used derivatizing agents for thiol and GSH assay based on the derivatizing reactions as shown in Scheme 1 [7–11]. As illustrated in Scheme 1A, DTNB reacts with a thiol compound (RSH) to form an adduct of thiol-5-thio-2-nitrobenzoic acid (RS-TNBA), and a concomitant release of one equivalent of 5-thio-2-nitrobenzoic acid (TNBA).
Glutathione, Oxidized Glutathione, and Mixed Disulfides in Biological Samples
2013, In Vitro Toxicity Indicators