Elsevier

Analytical Biochemistry

Volume 135, Issue 1, November 1983, Pages 16-21
Analytical Biochemistry

Activity stain for pyruvate decarboxylase in polyacrylamide gels

https://doi.org/10.1016/0003-2697(83)90724-8Get rights and content

Abstract

A method for the localization of pyruvate decarboxylase bands in polyacrylamide gels after electrophoresis using 1,2-dianilinoethane in dilute acetic acid as reagent for acetaldehyde formed from pyruvate by the holoenzyme is described. A white condensation product forms in the bands and precipitates within a few minutes. The more or less opaque bands can be viewed and photographed against a dark background and scanned in a densitometer. The detection limit is at about 10 mU pyruvate decarboxylase when the gels are bathed in the staining solution for 20 min. Several other methods were tested and failed to produce satisfactory results.

References (20)

  • A. Schellenberger et al.

    Angew. Chem

    (1968)
    A. Schellenberger et al.

    Int. ed. in English

    (1968)
  • M.M. Bradford

    Anal. Biochem

    (1976)
  • A. Görg et al.
  • H.G. Nimmo et al.

    Anal. Biochem

    (1982)
  • E. Steyn-Parvé et al.

    Z. Vitaminforsch

    (1944)
  • H. Holzer et al.

    Biochem. Z

    (1956)
  • A. Schellenberger

    Angew. Chem

    (1967)
    A. Schellenberger

    Int. ed. in English

    (1967)
  • K. Wetzel

    Planta

    (1932)
  • H. Lehmann et al.

    Eur. J. Biochem

    (1973)
  • H. Uhlemann et al.

    FEBS Lett

    (1976)
There are more references available in the full text version of this article.

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