Elsevier

Analytical Biochemistry

Volume 103, Issue 1, 15 March 1980, Pages 101-109
Analytical Biochemistry

Radioiodination of microgram quantities of ribosomal proteins from polyacrylamide gels

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Abstract

A method has been developed for radiolabeling small amounts of ribosomal proteins extracted from polyacrylamide gels with potassium [125]Iiodide. The procedure was used to label even those proteins which lack tyrosine and histidine residues by the modification of proteins with methyl p-hydroxybenzimidate. Specific radioactivities obtained range from 20,000 to 200,000 cpm/μg. The method has been used in the identification of eukaryotic ribosomal proteins from rabbit reticulocytes separated by polyacrylamide/sodium dodecyl sulfate gel electrophoresis.

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