Increase in cytoplasmic Ca2+ and stimulation of calcitonin secretion from human medullary thyroid carcinoma cells by the gastrin-releasing peptide

https://doi.org/10.1016/0006-291X(92)91702-RGet rights and content

Abstract

Examination was made of the effects of gastrin-releasing peptide (GRP) on human medullary thyroid carcinoma cells (TT cells). GRP stimulated calcitonin(CT) release in a concentration-dependent manner at 0.1–1000nmol/l. On adding forskolin along with GRP, CT release was greater than by GRP alone. The stimulatory effect of A23187 was not additive. Intracellular free calcium concentration ([Ca2+]i) was measured for individual TT cells loaded with fura-2. The addition of GRP caused a rapid and transient rise in [Ca2+]i in a concentration-dependent manner followed by a sustained increase in [Ca2+]i. In the medium without Ca2+, this sustained increase did not occur and the concentration of CT release from TT cells by GRP was reduced by approximately a half. GRP would thus appear to be importantly involved in the regulation of thyroid C cell function through modulation of [Ca2+]i.

References (19)

  • K. Yamaguchi et al.

    Metabolism

    (1984)
  • M.E. Sunday et al.

    Lab. Invest

    (1988)
  • J.H. Walsh et al.
  • F. Cuttitta et al.

    Nature

    (1985)
  • J.M. Westendorf et al.

    Endocrinology

    (1982)
  • S.L. Swope et al.
  • E. Rozengurt et al.
  • S. Weber et al.

    J. Clin. Invest

    (1985)
  • D.N. Carney et al.

    Cancer Res

    (1987)
There are more references available in the full text version of this article.

Cited by (5)

View full text