Increased sensitivity for detection of carcinogen-induced DNA repair with the chain terminator dideoxythymidine

https://doi.org/10.1016/0006-291X(78)91396-7Get rights and content

Abstract

The sensitivity for quantitation of DNA excision repair caused by a carcinogen is increased when the resynthesis of DNA is inhibited with dideoxythymidine (ddThd), a chain terminator. Incorporation of ddThd in primary liver cell cultures leaves discontinuities in the DNA chain at sites of reparative synthesis in response to benzo(a)pyrene [B(a)P] treatment. This is detectable by the accumulation of cellular DNA with reduced molecular weight on alkaline sucrose gradients. Up to 78 percent of the DNA in cells incubated with B(a)P and ddThd had greatly reduced molecular weight compared to control cells, whereas only 32 percent of the DNA in cells treated with B(a)P but not incubated with ddThd was affected similarly.

References (14)

  • D. Pettijohn et al.

    J. Mol. Biol

    (1964)
  • J. McCann et al.

    PNAS

    (1975)
  • J. McCann et al.

    PNAS

    (1976)
  • M.R. Atkinson et al.

    Biochem

    (1969)
  • H.L. Leffert et al.

    Nature

    (1977)
  • H.F. Stich et al.
  • M.W. Lieberman et al.

    Cancer Res

    (1971)
There are more references available in the full text version of this article.

Cited by (6)

  • Genetic toxicology

    1980, Food and Cosmetics Toxicology
  • Studies of DNA-strand breaks induced in human fibroblasts by chemical mutagens/carcinogens

    1979, Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
View full text