Evidence for an association of a ribonucleic acid with the purified, unactivated glucocorticoid receptor

https://doi.org/10.1016/0006-291X(86)91077-6Get rights and content

Abstract

The unactivated glucocorticoid-receptor complex (GRC) was purified from rat hepatic cytosol (approximately 4000-fold by specific activity) by a procedure developed in our laboratory. Following elution of unactivated GRC from DEAE-cellulose with a 0.05–0.5 M potassium phosphate gradient, a second gradient of 0.5–1.0 M potassium chloride was started. This gradient eluted material at 0.6 M potassium chloride that incorporated [32P] in vivo and stained with ethidium bromide. A predominant ethidium bromide stained band of 100–110 nucleotides was observed. The presence of this material was dependent on the presence of highly purified GRC since presaturation of cytosol with 50 μM unlabeled triamcinolone acetonide (TA) precluded the appearance of this material. Experiments with partially purified GRC from CEM-C7 cells incubated in vivo with [14C]uridine indicated that the material eluted at 0.6 M potassium chloride incorporated [14C]-uridine. Collectively, these data suggest that a RNA is associated with the purified, unactivated form of the GRC.

References (19)

  • G.P. Rossini

    J. Steroid Biochem

    (1985)
  • J.L. Tymoczko et al.

    J. Steroid Biochem

    (1982)
  • J.L. Tymoczko et al.

    Arch. Biochem. Biophys

    (1984)
  • P. Grandics et al.

    J. Biol. Chem

    (1984)
  • P. Grandics et al.

    Biochem. Biophys. Res. Commun

    (1984)
  • I.V. Economidis et al.

    FEBS Letts

    (1985)
  • S. Liao et al.

    J. Biol. Chem

    (1980)
  • M.T. Chong et al.

    J. Biol. Chem

    (1982)
  • J.L. Tymoczko et al.

    Endocrinology

    (1983)
There are more references available in the full text version of this article.

Cited by (17)

View all citing articles on Scopus
+

Present address: Department of Pharmacology, Hershey Medical Center, The Pennsylvania State University, Hershey, PA, 17033

§

Present address: The Department of Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242

Address this author for communication.

View full text