Sustained cell culture,☆☆

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Abstract

Gas exchange, introduction of nutrients, and removal of waste products can be regulated in a new device for continuous sustained culture of cells in suspension.

Strain L, clone 929, fibroblasts; HeLa cells; and strains KB, and Chang, liver cells were successfully propagated in this device.

Ehrlich ascites cells have been propagated in vitro for the time.

References (30)

  • S. Barban et al.

    J. Biol. Chem

    (1956)
  • H. Eagle

    J. Biol. Chem

    (1955)
  • H. Eagle

    Arch. Biochem. and Biophys

    (1956)
  • M. Jones et al.

    Exptl. Cell Research

    (1956)
  • H.A. Krebs

    Biochim. et Biophys. Acta

    (1950)
  • T. Barka et al.

    Acta Morphol. Acad. Sci. Hung

    (1955)
  • M.T. Burrows

    Am. J. Phys

    (1924)
  • R.S. Chang
  • H. Eagle

    Science

    (1955)
  • H. Eagle
  • W.R. Earle et al.

    Ann. N.Y. Acad. Sci

    (1956)
  • W.R. Earle et al.

    J. Natl. Cancer Inst

    (1950)
  • W.R. Earle et al.

    J. Natl. Cancer Inst

    (1951)
  • V. Evans et al.

    Cancer Research

    (1956)
  • C.L. Gemmill et al.

    Bull. Johns Hopkins Hosp

    (1940)
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    This work was supported by a grant from the Damon Runyon Memorial Fund.

    ☆☆

    This report was submitted to the Faculty of Pure Science, Columbia University, by Kenneth S. McCarty in partial fulfillment of the requirements for the degree of Doctor of Philosophy.

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