A simple and rapid method for the determination of the number of 3H-ouabain binding sites in biopsies of skeletal muscle

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Summary

Based on vanadate facilitation of 3H-ouabain binding to Na-K-ATPase, a method has been developed allowing the measurement of the number of 3H-ouabain binding sites in small rat muscle biopsies (2–14 mg). Comparison with results obtained using intact fibres showed close agreement over the wide range of values for 3H-ouabain binding sites (100–900 pmol per g wet weight) associated with variations in age, thyroid status and K-deficiency.

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