Mechanisms for the stimulatory and inhibitory effects of carbamoylcholine on canine gastric D-cells

https://doi.org/10.1016/S0006-291X(87)80098-0Get rights and content

We have previously reported that carbamoylcholine (carbachol), a recognized inhibitor of somatostatin release from D-cells, can act as stimulant following pretreatment of cells with pertussis toxin. In the present studies we have observed that pertussis toxin reverses the inhibitory effects of carbachol on D-cell stimulated with either cAMP or 12-0-tetradecanoyl-phorbol 13-acetate. Furthermore, the stimulatory effects of carbachol on D-cells pretreated with pertussis toxin potentiated the actions of pentagastrin without further enhancing the release of 3H-inositol trisphosphate from prelabeled cells. These studies suggest that carbachol exerts its inhibitory effects on D-cells via pertussis toxin sensitive guaninine nucleotide binding proteins at a point distal to the activation of different signal transduction mechanisms and that the stimulatory effects of carbachol are mediated by mechanisms that are independent of membrane phosphoinositide turnover.

References (9)

  • ChibaT. et al.

    J. Biol. Chem.

    (1987)
  • KuroseH. et al.

    J. Biol. Chem.

    (1983)
  • LindemannJ.P. et al.

    J. Biol. Chem.

    (1985)
  • YamadaT. et al.

    Am. J. Physiol.

    (1984)
There are more references available in the full text version of this article.

Cited by (0)

View full text