Enzymatic sulfation of salivary mucins: Structural features of 35S-labeled oligosaccharides

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Summary

Sulfotransferase activity catalyzing the transfer of sulfate ester group from 3′-phosphoadenosine-5′-phosphosulfate to salivary mucins was found associated with Golgi-rich membrane fraction of rat submandibular salivary glands. Alkaline borohydride reductive cleavage of the synthesized 35S-labeled glycoprotein led to the liberation of the label into reduced acidic oligosaccharides. Most of the label was found incorporated in four oligosaccharides. These were identified as sulfated tri-, penta-, hepta- and nonasaccharides. The trisaccharide was characterized as SO3H,6GlcNAcβl, 3Galβl,3GalNAc-ol, the pentasaccharide as SO3H,6GlcNAcβl,3Galβl, 4GlcNAcβl,3(NeuAcα2,6) GalNAc-ol and heptasaccharide as SO3H,6Galβ1,4GlcNAcβ1,6(Fucα1) 2Galβl,4GlcNAcβl,3Galβl,3)GalNAc-ol.

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