Studies on urea synthesis, insulin degradation and phagocytosis by isolated rat kupffer cells*

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Summary

Isolated Kupffer cells synthesize urea from a variety of added amino acids at a rate comparable to that of hepatocytes. Urea synthesis was determined both by enzymic product analysis and by the incorporation of labeled bicarbonate into urea. Studies on uptake of radioactive insulin showed that both hepatocytes and Kupffer cells bind insulin to almost the same extent. However, much higher degradation of insulin was found with Kupffer cells than with hepatocytes. Labeled insulin-anti-insulin serum (I-AIS) complex is neither bound nor taken up by hepatocytes. Kupffer cells, on the other hand, removed the complex in a time-dependent manner from the medium and partially degraded it.

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*

This work was supported by grants from the deutsche Forschungsgemeinschaft, Bonn-Bad Godesberg, Federal Republic of Germany, through Forschergruppe “Lebererkrankungen”.

**

On sabbatical leave from Department of Pharmacology, Indiana University Medical School, Indianapolis, Indiana 46202, U.S.A.

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