Reversible G1 arrest of a human Burkitt lymphoma cell line(Raji) induced by tunicamycin

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Summary

The effects of tunicamycin on the growth of a human Burkitt lymphoma cell line(Raji) were examined. The growth of cells was reversibly inhibited in the presence of tunicamycin (<0.05 μg/ml). Viability of cells treated with less than 1.0 μg/ml of tunicamycin was maintained at more than 90 % for 3 days at least. Analysis of tunicamycin-treated cells by flow-microfluorometry showed that cells were clearly arrested in the GI phase of the cell cycle. The components that were pulse-labeled with [3H]glucosamine after removal of tunicamycin were analysed by urea-sodium dodecyl sulfate polyacryl amide gel electrophoresis. The biosynthesis of glycoproteins with apparent molecular weights of 107 000, 44 000 and 38 000 were restored prior to the reinitiation of DNA synthesis. It is probable that these glycoproteins are needed for the cell to traverse G1 phase.

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