Cleavage of phycocyanobilin from C-phycocyanin

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Abstract

The bile pigment chromophore of C-phycocyanin, phycocyanobilin, was cleaved from the protein by several procedures. Comparison of the crystalline phycocyanobilin dimethyl ester by chemical, spectral, and chromatographic properties was made. The pigment was found to be identical by all procedures. Highest yields of phycocyanobilin were obtained by the methanol cleavage. The conversion of phycocyanobilin to mesobiliverdin by KOH was confirmed.

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