Reactivity of artificial substrates for prenyltransferase

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Abstract

Four compounds out of 6 new allylic pyrophosphates synthesized were found to act as artificial substrates for pig liver prenyltransferase (dimethylallylpyrophosphate:isopentenylpyrophosphate dimethylallyltransferase, EC 2.5.1.1). These were cyclopentylideneethyl, cyclohexylideneethyl, trans-3-ethyl-2-hexenyl, and cis-3-ethyl-2-hexenyl pyrophosphates. 2-Heptenyl and 2-octenyl pyrophosphates which have no substituent at the 3 position were inactive. The reactivities of these artificial substrates including 4 known compounds, trans- and cis-3-methyl-2-hexenyl pyrophosphates, and trans- and cis-3-methyl-2-heptenyl pyrophosphates were compared in terms of Km and vmax, and it was found that the trans structure was favored for the binding to the enzyme.

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