Biochimica et Biophysica Acta (BBA) - Biomembranes
Regular paperDielectric analysis of mitochondria isolated from rat liver II. Intact mitochondria as simulated by a double-shell model☆
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Cited by (54)
Electrophysical sensor systems for in vitro monitoring of bacterial metabolic activity
2022, Biosensors and Bioelectronics: XDielectric properties of isolated adrenal chromaffin cells determined by microfluidic impedance spectroscopy
2018, BioelectrochemistryCitation Excerpt :The hepatocyte cell membrane capacitance derived in the study by Raicu et al. agreed with that calculated using independent observations, namely electron microscopy measurements for cell surface area estimation. Furthermore, the mitochondrial membrane capacitance of 0.34 μF/cm2 in this study deviated only by 12.8% from the mitochondrial membrane capacitance value obtained using dielectric spectroscopy of hepatocytic mitochondrial suspensions [1]. Irimajiri et al. measured dielectric spectrum of lymphoma cells and compared the vesicular and the double shell models for modeling the measured dielectric spectrum.
Dielectric response of shelled toroidal particles carrying localized surface charge distributions. The effect of concentric and confocal shells
2014, BioelectrochemistryCitation Excerpt :Radiowave dielectric relaxation spectroscopy is a powerful tool of increasing importance in the study of mechanisms of interactions of electric fields with biological objects, mainly biological cells, making possible the characterization of the passive electrical properties of the cell membrane and, in most cases, of the cytosol and the extracellular medium [1–8]. This methodology has been widely used to characterize a variety of biological systems, ranging from erythrocytes to lymphocytes in normal and pathological conditions [9], to Escherichia coli cells [10], intact mitochondria (isolated from rat liver) [11], mitoplasts [12] and different cell lines, such as MadinDarby Canine Kidney Epithelial [MDCK] line and Bone Marrow derived Pre-Osteoblastic [MBA] line [13], up to various biological tissues [14–16]. The dielectric response of a biological cell induced by an external electric field is characterized by a rather complex spectrum composed of different relaxation regions, corresponding to different mechanisms at a molecular level occurring in the system [17,18].
On the dielectric relaxation of biological cell suspensions: The effect of the membrane electrical conductivity
2011, Colloids and Surfaces B: BiointerfacesCitation Excerpt :In any case, in an intermediate range of values, between σs = 10−5 to 10−3 mho/m, the changes in the dielectric, Δϵ, and conductivity, Δσ, increments are large enough to allow, at least in principle, a meaningful evaluation of the membrane conductivity. In the light of the two-shell model, the effect of the membrane conductivity has been also addressed by Asami and Irimajiri [14] in the analysis of the dielectric response of intact mitochondria, isolated from rat liver. These authors based their analysis on the assumption that for the conductivities of both the membranes (the outer membrane σso and the inner membrane σsi), the following conditions hold, σso = ≤10−6σm, i.e., the two conductivities are practically zero.
Examination of the induced potential gradients across inner and outer cellular interfaces in a realistic 3D cytoplasmic-embedded mitochondrion model
2010, Journal of Electroanalytical ChemistryEffects of oscillatory electric fields on internal membranes: An analytical model
2008, Biophysical JournalCitation Excerpt :The induced membrane potential across arbitrarily shaped cellular membranes with mobile surface charges was calculated by Prodan and Prodan in describing the dielectric behavior of living cell suspensions (24). Asami and Irimajiri (25) employed a double-shell model to extract electrical parameter values from dielectric response measurements of intact mitochondria. More recently, Kotnik and Miklavčič calculated the ac transmembrane voltage induced on spheroidal cells (26) and across internal membranes, using a double-shell model for application to cells exposed to nanosecond-duration pulsed electric fields (27).
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For Paper I see Ref. 1.