Conformation and packing properties of membrane lipids: The crystal structure of sodium dimyristoylphosphatidylglycerol

doi:10.1016/0005-2736(87)90358-0

Biochimica et Biophysica Acta (BBA) - Biomembranes

Volume 896, Issue 1, 9 January 1987, Pages 77-88

https://doi.org/10.1016/0005-2736(87)90358-0 Get rights and content

Abstract

The conformation and molecular packing of sodium 1,2-dimyristoyl-sn-glycero-phospho-trac-glycerol (DMPG) have been determined by single crystal analysis (R = 0.098). The lipid crystallizes in the monoclinic spacegroup P2₁ with the unit cell dimensions a = 10.4, b = 8.5, $c = 45.5 A ̊$ and β = 95.2°. There are two independent molecules (A and B) in the asymmetric unit which with respect to configuration and conformation of their glycerol headgroup are mirror images. The molecules pack tail to tail in a bilayer structure. The phosphoglycerol headgroups have a layer-parallel orientation giving the molecules an L-shape. At the bilayer surface the (-) phosphoglycerol groups are arranged in rows which are separated by rows of (+) sodium ions. Laterally the polar groups interact by an extensive network of hydrogen, ionic and coordination bonds. The packing cross-section per molecule is 44.0 Å². The hydrocarbon chains are tilted (29°) and have opposite inclination in the two bilayer halves. In the chain matrix the chain planes are arranged according to a so far unknown hybride packing mode which combines the features of T_| and O_⊥ subcells. The two fatty acid substituted glycerol oxygens have mutually a — synclinal rather than the more common + synclinal conformation. The conformation of the diacylglycerol part of molecule A and B is distinguished by an axial displacement of the two hydrocarbon chains by four methylene units. This results in a reorientation of the glycerol back bone and a change in the conformation and stacking of the hydrocarbon chains. In molecule A the β-chain is straight and the γ-chain is bent while in molecule B the chain conformation is reversed.

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Conformation and packing properties of membrane lipids: The crystal structure of sodium dimyristoylphosphatidylglycerol

Abstract

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Chem. Phys. Lipids

Biochim. Biophys. Acta

Progr. Chem. Fats Lipids

Chem. Phys. Lipids

Biochim. Biophys. Acta

J. Mol. Biol.

Chem. Phys. Lipids

J. Mol. Biol.

Chem. Phys. Lipids

Chem. Phys. Lipids

Biochem. J.