Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
Studies with hydroxyurea: I. The reversible inhibition of bacterial DNA synthesis and the effect of hydroxyurea on the bactericidal action of streptomycin
References (59)
- et al.
Biochim. Biophys. Acta
(1965) - et al.
Biochim. Biophys. Acta
(1964) - et al.
J. Biol. Chem.
(1951) J. Mol. Biol.
(1961)- et al.
J. Mol. Biol.
(1962) - et al.
Biochim. Biophys. Acta
(1959) - et al.
J. Mol. Biol.
(1959) - et al.
J. Mol. Biol.
(1964) - et al.
Biochem. Biophys. Res. Commun
(1959) - et al.
Biochim. Biophys. Acta
(1963)
Biochem. Biophys. Res. Commun.
Biochim. Biophys. Acta
J. Mol. Biol.
Biochem. Biophys. Res. Commun.
Biochim. Biophys. Acta
Biochem. Biophys. Res. Commun.
Biochim. Biophys. Acta
J. Mol. Biol.
Biochem. Pharmacol.
Biochim. Biophys. Acta
J. Mol. Biol.
Virology
Biochim. Biophys. Acta
J. Mol. Biol.
J. Bacteriol.
J. Bacteriol.
Anal. Chem.
Biochem. J.
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Multiple C-terminal tails within a single E coli SSB homotetramer coordinate DNA replication and repair
2013, Journal of Molecular BiologyCitation Excerpt :E. coli cells expressing either wt SSB or SSB-LD-Drl were grown in the presence of the DNA damaging agents hydroxyurea (HU) and nitrogen mustard [N(CH2CH2Cl)3 or HN2] or exposed to UV irradiation. HU is an inhibitor of ribonucleotide reductase, and treatment of E. coli results in depletion of dNTP pools leading to DNA double-strand breaks near replication forks [66,67], whereas HN2 inhibits DNA replication by covalently cross-linking the two DNA strands [68]. Exposure of cells to UV irradiation leads to formation of DNA breaks, base damage and UV sensitivity [69].
Induction of competence for genetic transformation by antibiotics: Convergent evolution of stress responses in distant bacterial species lacking SOS?
2012, Current Opinion in MicrobiologyCitation Excerpt :Thus, exposure to bleomycin, a natural antibiotic which provokes DNA DSB and induces the SOS response (Table 1), does not trigger L. pneumophila competence [13•]. On the other hand, hydroxyurea, a small molecule inhibitor of class I ribonucleotide reductase commonly used to study DNA damage-independent replication-fork stalling [34,35], induces competence in both L. pneumophila [13•] and S. pneumoniae (Vanessa Khemici, JPC, PP, unpublished observations). Furthermore, the finding that high FQ doses failed to induce competence in S. pneumoniae [12••], H. pylori [21] and L. pneumophila [13•], while lower FQ concentrations did, is consistent with induction resulting from replication-fork stalling as low FQ concentrations produce reversible ‘frozen’ gyrase intermediates, which block the DNA polymerase [36], whereas high FQ doses induce DSB.
Platinum-DNA interstrand crosslinks: Molecular determinants of bending and unwinding of the double helix
2012, Journal of Inorganic BiochemistryCitation Excerpt :In addition, eukaryotic DNA polymerases β, ζ, γ, and η bypass oxaliplatin adducts more readily than cisplatin adducts and the misincorporation frequency of DNA polymerase β is slightly greater with oxaliplatin adducts than with those of cisplatin [3–5]. For cisplatin, a number of observations point to DNA as the critical cellular target: filamentous growth of bacteria following cisplatin treatment [6–8], induction of lambda prophage in lysogenic bacteria [9], inactivation of hemophilus-transforming DNA [10], and particularly lethal effects on Escherichia coli mutants having deficient DNA repair systems [11, 12]. For oxaliplatin, a tumor-killing mechanism triggered by an initial DNA attack is assumed as well, although the evidence is less elaborated in this case.
Cyanide, Peroxide and Nitric Oxide Formation in Solutions of Hydroxyurea Causes Cellular Toxicity and May Contribute to Its Therapeutic Potency
2009, Journal of Molecular BiologyCitation Excerpt :HU was used to examine the role of DNA replication in TLD, but results were conflicting.10,28 In earlier studies, 100 mM HU brought a complete protection against TLD;10 in a more recent report, 75 and 100 mM HU offered no protection, while 200 mM HU did.28 The latter authors reported, though, that all HU concentrations blocked DNA synthesis completely, proposing that it is HU's modest effect on RNA synthesis, which is responsible for the observed protection.28
The Influence of Cisplatin on the Gas-Phase Dissociation of Oligonucleotides Studied by Electrospray Ionization Tandem Mass Spectrometry
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