Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
Volume 834, Issue 1, 27 March 1985, Pages 8-22
[1-14C]Arachidonic acid incorporation into glycerolipids and prostaglandin synthesis in peritoneal macrophages: effect of chloramphenicol
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2011, Nutrition ResearchCitation Excerpt :After 2 weeks on the experimental diets, a time length demonstrated to induce an appropriate and immune effect [7,16]; using a 21-gauge needle, 1 mg of opsonized zymosan (Sigma, St Louis, Mo, USA) in 0.5 mL of saline (9 g NaCl/L) was injected into the peritoneum of the mice to stimulate macrophages and induce eicosanoid biosynthesis [17]. Opsonized zymosan was prepared by the method of Maroussem et al [18] as modified by Broughton et al [7]. Briefly, zymosan was boiled at 100°C in distilled water at 10 g/L for 1 hour.
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