The hexa- and pentapeptide extension of proalbumin II. Processing of specific antibodies against the synthetic hexapaptide

https://doi.org/10.1016/0005-2795(81)90116-1Get rights and content

Abstract

The chemically synthesized proalbumin hexapeptide was coupled to rabbit albumin with carbodiimide. Subsequently rabbits were immunized by subcutaneous injection of the conjugate. After 5 weeks the rabbits had developed antibodies against the hexapeptide, which could be detected by immunodiffusion. A sensitive enzyme-linked immunosorbent assay was developed. Using the hexapeptide and other very similar peptides as antigens, a high specificity of the antibodies against the proalbumin hexapeptide was found.

References (15)

  • Th. Peters et al.

    J. Biol. Chem.

    (1980)
  • Chr. Birr et al.

    Biochim. Biophys. Acta

    (1981)
  • K. Weigand et al.

    Comp. Biochem. Physiol.

    (1980)
  • W. Bommeli et al.

    Vet. Immunol. Immunopathol.

    (1980)
  • Th. Peters
  • P.S. Quinn et al.

    Biochem. J.

    (1978)
  • L. Ornstein

    Ann. N.Y. Acad. Sci.

    (1964)
There are more references available in the full text version of this article.

Cited by (15)

  • Peptide Antibodies: New Tools for Cell Biology

    1986, International Review of Cytology
  • A modified ELISA technique for anti-hapten antibodies

    1982, Journal of Immunological Methods
View all citing articles on Scopus
View full text