Conformation and packing properties of phosphatidic acid: The crystal structure of monosodium dimyristoylphosphatidate

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Abstract

The conformation and molecular packing of monosodium 1,2-dimyristoyl-sn-glycerophosphate (DMPA) has been determined by single crystal analysis (R = 0.107). The lipid crystallizes in the space group P21 with unit cell dimensions: a = 5.44, b = 7.95, c = 43.98 Å and β = 114.2°. The two molecules of the unit cell are related by a two-fold screw axis and pack tail-to-tail in a bilayer structure. The monosodium phosphate group packs with rather a small cross-section (24 Å2) relative to the two hydrocarbon chains. This unbalance in packing cross-section is overcome by an interdigitation of the phosphate head groups of adjacent bilayers and the formation of a single, common phosphate group layer at the bilayer interfaces. The phosphate groups are linked by hydrogen bonds to linear strands which laterally are separated by strands of sodium ions. The conformation of the molecules differs from that of other phospholipids. The glycerol chain is oriented parallel (instead of perpendicular) to the layer surface and the parallel stacking of the hydrocarbon chains is achieved by a bend of the γ-chain (instead of the β-chain). Otherwise the conformation of the glycerol dicarboxyl ester group displays the same preferred features as generally found in glycerophospholipids. The hydrocarbon chains pack according to the triclinic (T) packing mode. The interaction and packing principles of the phosphate head group are discussed in relation to the structural behaviour of phosphatidic acid.

References (33)

  • E.G. Lapetina et al.

    J. Biol. Chem.

    (1981)
  • S.D. Shukla et al.

    Biochem. Biophys. Res. Commun.

    (1982)
  • M. Koter et al.

    Biochim. Biophys. Acta

    (1978)
  • R. Sundler et al.

    Biochim. Biophys. Acta

    (1981)
  • A.J. Verkleij et al.

    Biochim. Biophys. Acta

    (1982)
  • H. Eibl et al.

    Biochim. Biophys. Acta

    (1979)
  • A. Blume et al.

    Biochim. Biophys. Acta

    (1979)
  • H. Eibl et al.

    Biophys. Chem.

    (1979)
  • H. Eibl
  • H. Träuble et al.

    Biophys. Chem.

    (1976)
  • S. Abrahamsson et al.

    Prog. Chem. Fats Other Lipids

    (1978)
  • H. Hauser et al.

    Biochim. Biophys. Acta

    (1981)
  • I. Pascher et al.

    J. Mol. Biol.

    (1981)
  • I. Pascher et al.

    Chem. Phys. Lipids

    (1977)
  • I. Pascher et al.

    Chem. Phys. Lipids

    (1982)
  • Cited by (0)

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