Short sequence-paper
A conserved sequence in tRNA and rRNA promoters of Lactococcus lactis

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Abstract

A tRNA operon (trnA) from Lactococcus lactis consisting of seven tRNA genes and a 5S rRNA gene was cloned and sequenced. Promoter-fusion of the trnA promoter to a promoter-less β-galactosidase gene of Leuconostoc mesenteroides resulted in high levels of β-galactosidase activity in L. lactis. Searching for sequences with similarity to the sequence of the promoter region revealed a consensus sequence of promoters preceeding rRNA operons and tRNA operons from Lactococcus species including a not previously described conserved sequence (AGTT).

References (18)

  • C. Chiaruttini et al.

    J. Mol. Biol.

    (1993)
  • G. Duester et al.

    Cell

    (1982)
  • R. Gourse et al.

    Cell

    (1986)
  • T.M. Henkin et al.
  • M.C. Graves et al.

    J. Biol. Chem.

    (1986)
  • E.F. Wawrousek et al.

    J. Biol. Chem.

    (1984)
  • A. Rich
  • D. Nilsson et al.

    Mol. Gen. Genet.

    (1992)
  • T. Beresford et al.

    J. Gen. Microbiol.

    (1993)
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