Characterization of two classes of non-fixing mutants of pea plants (Pisum sativum L.)

doi:10.1016/0168-9452(93)90079-F

Plant Science

Volume 95, Issue 1, 1993, Pages 55-66

https://doi.org/10.1016/0168-9452(93)90079-F Get rights and content

Abstract

Seven (Nod⁺Fix⁻) mutants of pea cv. Frisson were isolated following ethylmethane sulphonate mutagenesis. Histological, fluorescence and molecular approaches have been used to characterized symbiotic phenotypes and to classify the mutants. In all cases, Rhizobium infection occurred via root hairs and resulting nodules had a typical indeterminate structure. However, mutants could be broadly classified into two group on the basis of nodule development and the level of nodule-specific gene transcripts. In the first groups, comprising P12, P58, P61 and P63 lines, nodule development was approximately normal but was followed by premature senescence. In the second group comprising P59, P60 and P62, mutant lines showed an early arrest of nodule development. Developmentally-defective root hairs were also observed on roots of mutant P62.

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Phenotypic characterization of two nodulation mutants (R69 and R99) of Phaseolus vulgaris L. was undertaken. The nodule anatomy of R69 ( $Nod^{+} Fix^{−}$ ), was examined and compared to its wild-type parent, OAC Rico. The arbuscular mycorrhizal (AM) status of R69 and R99 (Nod⁻) was also compared to OAC Rico. Small whitish growths resembling nodules were produced on the roots of R69 in response to inoculation with Rhizobium leguminosarum biovar phaseoli. These nodule-like structures were variable in shape and occasionally had lateral roots growing from them. A layer of crystalline inclusions (possibly calcium oxalate) occurred between the periderm and parenchyma cells in the mutant nodules. A central infected zone was not present and the vascular system occupied the centre of the nodule instead of the periphery, as seen in wild-type nodules. Given the distinctive phenotype of the nodules of R69, they were designated Pvb⁻ (peripheral vascular bundles, minus). The Nod⁻ mutant, R99, could establish a symbiosis with AM (Myc⁺), however, R69 was resistant to infection by AM (Myc⁻). Appressoria would form on the roots and internal hyphae would start to develop, but these would fail to develop into a normal AM symbiosis. We suggest that specific anatomical characteristics of the $Nod^{+} Fix^{−}$ and Myc⁻ phenotypes in R69 supports the hypothesis of a common regulation of the development of the two symbioses.
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Genetics and molecular biologyCharacterization of two classes of non-fixing mutants of pea plants (Pisum sativum L.)

Abstract

Plant Sci. Lett.

Plant Sci.

Plant Sci.

Anal. Biochem.

Cell

Plant Sci.

Physiol. Plant Pathol.

Cell

Correlation between ultrastructural differentiation of bacteroids and nitrogen fixation in alfalfa nodules

J. Bacteriol.

cDNA cloning and developmental expression of pea nodulin genes

Plant Mol. Biol.

Nodulin function and nodulin gene regulation in root nodule development

Three pea mutants with an altered nodulation studied by genetic analysis and grafting

J. Plant Physiol.

Induced symbiosis mutants of pea (Pisum sativum) and sweetclover (Melilotus alba annua)

Plant Sci.

Sym 13, a gene conditioning ineffective nodulation in Pisum sativum L.

Plant Physiol.

Nodulation and nitrogen fixation mutants of pea, Pisum sativum L.

Theor. Appl. Genet.

New symbiotic mutants of pea (Pisum sativum L.) affecting either nodule initiation or symbiosome development

Symbiosis

Isolation and properties of soybean (Glycine max (L.) Merr.) mutants that nodulate in presence of high nitrate concentrations

Selection and initial characterization of partially nitrate tolerant nodulation mutants of soybean

Plant Physiol.

Genetics and molecular biology
Characterization of two classes of non-fixing mutants of pea plants (Pisum sativum L.)