Biochimica et Biophysica Acta (BBA) - General Subjects
Generation of one set of monoclonal antibodies specific for a-pathway ganglio-series gangliosides
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2016, Biochemistry and Biophysics ReportsCitation Excerpt :Here, we first demonstrated that co-administration of recombinant GM2A could significantly enhance the reduction of intracellular GM2 accumulated in TSD fibroblasts mediated by modB in the culture system. Anti-GM2 mouse monoclonal antibodies (GMB28: IgM) were established previously [8]. The CHO-K1 cell line was provided by the RIKEN BioResource Center (Ibaraki, Japan).
Gangliosides of the Vertebrate Nervous System
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2016, Chemistry and Physics of LipidsCitation Excerpt :This method was also applied to nucleated cells, showing 80% of gangliosides GM1, GM3 and GD1a in the plasma membrane outer leaflet in N18 cells, murine neuroblastoma cell line (Miller-Podraza et al., 1982) and over 90% of galactosylceramide in the outer leaflet of myelin membrane (Linington and Rumsby, 1980). Several toxins and antibodies bind specific glycolipids, such as cholera toxin (binds ganglioside GM1) (Holmgren et al., 1973), Shiga toxin (Verotoxin) (binds globoside globotriaosylceramide (Gb3)) (Jacewicz et al., 1986), anti-GM1 antibody (Kotani et al., 1992), anti-ganglioside GM3 antibody (Kotani et al., 1992), and anti-phosphatidylglucoside antibody (Nagatsuka et al., 2003; Yamazaki et al., 2006). Phosphatidylglucoside is a recently identified glycophospholipid in which glucose is conjugated to phosphatidic acid (Takahashi et al., 2012).
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