Identity of blue pigments obtained from different tissues of the sculpin, Pseudoblennius percoides Günther

doi:10.1016/0305-0491(76)90177-2

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry

Volume 55, Issue 1, 1976, Pages 85-87

https://doi.org/10.1016/0305-0491(76)90177-2 Get rights and content

Abstract

1.
1. Blue chromoproteins were isolated from the skin, muscle, digestive tract and eggs of the sculpin, Pseudoblennius percoides Günther.
2.
2. They showed the same absorption spectrum and almost the identical molecular weights (38,000–42,000) and isoelectric points (3.4–3.7).
3.
3. The identity of these chromoproteins was confirmed by isoelectric focussing with mixed samples.
4.
4. Their chromophores were identified as being solely biliverdin IX₂.
5.
5. It may be concluded that one and the same biliverdin-protein exists in the different tissues of this fish.

References (13)

P.S. Low et al.
Bile pigments in the blood serum of fish from the family Cottidae
Comp. Biochem. Physiol.
(1974)
C.J.O.R. Morris
Thin-layer chromatography of proteins on Sephadex G-100 and G-200
J. Chromatogr.
(1964)
L. Abolinš
The preparation and identification of the chromoprotein Crenilabrus blue
Pubbl. Staz. Zool. Napoli
(1970)
L. Abolinš et al.
Über die farbgebende Gruppe von Crenilabrus-Blau
Experientia
(1966)
K.-B. Augustinsson
Electrophoresis studies on blood plasma esterases
Acta Chem. Scand.
(1959)
J.L. Bada
A blue-green pigment isolated from blood plasma of the Arctic sculpin (Myoxocephalus scorpioides)
Experientia
(1970)

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Cited by (14)

Green-blood pigmentation in lizards
1994, Comparative Biochemistry and Physiology -- Part A: Physiology
The green pigment in the plasma of the scincid lizard genus Prasinohaema is identified as the bile pigment biliverdin. Concentrations of biliverdin in the plasma of P. flavipes, P. prehensicauda and P. virens are 714 ± 79 μmol/1 (mean ± one standard deviation), 1020 ± 624 μmol/1 and 819 ± 89 μmol/1, respectively. These values represent the highest concentration of plasma biliverdin measured for any organism and are the first report of non-pathological biliverdin accumulation in amniotes. We review the literature for fish species with high concentrations of plasma biliverdin and pathological biliverdin accumulation in humans; we find that Prasinohaema species have plasma biliverdin concentrations approximately 1.5–30 times greater than fish species with green blood plasma and 40 times greater than humans with green jaundice.
The blue-green blood plasma of marine fish
1990, Comparative Biochemistry and Physiology -- Part B: Biochemistry and
- 1.
  1. The blue-green coloration of the blood plasma in some marine fishes, which is attributed to a protein bound tetrapyrrol (biliverdin), is an anomaly in vertebrates.
- 2.
  2. Recent studies have shown that biliverdin not only occurs in many fish, but is also present in the blood of tobacco hornworm, the wings of moth and butterfly, the shell of bird eggs, the serum and egg of frog, the placenta of dog and in the blood of humans suffering from hepatic diseases.
- 3.
  3. In this review, we begin with a historical account of the description of the presence of blue-green blood plasma in fish, and then consider the biochemistry, metabolism, physiology, and the ecological implications of biliverdin in fish.
- 4.
  4. A comparative description of the occurrence of biliverdin in fish and other animals is presented.
- 5.
  5. The mechanism of accumulation of biliverdin in fish blood and its evolutionary significance are also considered. It is suggested that this process may serve as a useful model for further research on bile pigment metabolism in other animals.
Characteristics of a bili-protein complex in the serum of the stargazer fish Genyagnus novazelandiae: Uranoscopidae) and its possible role in camouflage
1989, Comparative Biochemistry and Physiology -- Part B: Biochemistry and
- 1.
  1. The serum of the stargazer fish contains an 80,000 mol. wt protein which has firmly attached to it a blue-green bile pigment which can be removed only under harsh solution conditions.
- 2.
  2. Separation of the bile pigment from the protein using acid-methanol treatment followed by spectroscopic and chemical investigations identify the bile pigment present as biliverdin.
- 3.
  3. The nature of the biliverdin-protein complex is compared with that seen in some other species and a biological role in camouflage is proposed.
Identification of the blue-green chromophore of an abundant biliprotein from the haemolymph of Artemia
1984, Comparative Biochemistry and Physiology -- Part B: Biochemistry and
- 1.
  1. The haemolymph of adult Artemia is frequently coloured blue-green by a glycoprotein complex, artemocyanin I.
- 2.
  2. Artemocyanin II (M_r 1.2 × 10⁶), a structurally modified form of artemocyanin I purified from Artemia homogenates, has a blue chromophore which has been identified as a bile pigment related to biliverdin.
- 3.
  3. It is suggested that the bile pigment is derived from the turnover of haemoglobin and is bound to artemocyanin for transport and excretion.
Comparison of pigments in the integument of cobalt, albino, and normal rainbow trout, Salmo gairdnerii irideus
1981, Comparative Biochemistry and Physiology -- Part B: Biochemistry and
- 1.
  1. Integumental pigments were analyzed for the cobalt variant and compared with those of albino and normal rainbow trout.
- 2.
  2. The contents of carotenoids and pteridines per 100 g integument in cobalt, albino, and normal rainbow trout were 0.12, 0.26, and 0.63 mg for carotenoids and 3.3, 2.7, and 0.51 mg for pteridines, respectively.
- 3.
  3. The main pteridine was identified as being isoxanthopterin in common among the three kinds of rainbow trout.
- 4.
  4. Eumelanin was detected in the integument of cobalt and normal rainbow trout and the contents were determined to be 48 and 150 mg/100 g, respectively.
- 5.
  5. The characteristic cobalt blue coloration may originate mainly in the differences in the content and arrangement of isoxanthopterin and melanin in the coloring tissue.
The weaker sex: Male lingcod (Ophiodon elongatus) with blue color polymorphism are more burdened by parasites than are other sex–color combinations
2021, PLoS ONE

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^∗: Present address: Sch. of Fish. Sci., Kitasato Univ., Sanriku, Iwate, Japan.

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Comparative Biochemistry and Physiology Part B: Comparative Biochemistry

Abstract

References (13)

Bile pigments in the blood serum of fish from the family Cottidae

Comp. Biochem. Physiol.

Thin-layer chromatography of proteins on Sephadex G-100 and G-200

J. Chromatogr.

The preparation and identification of the chromoprotein Crenilabrus blue

Pubbl. Staz. Zool. Napoli

Über die farbgebende Gruppe von Crenilabrus-Blau

Experientia

Electrophoresis studies on blood plasma esterases

Acta Chem. Scand.

A blue-green pigment isolated from blood plasma of the Arctic sculpin (Myoxocephalus scorpioides)

Experientia

Cited by (14)

Green-blood pigmentation in lizards

The blue-green blood plasma of marine fish

Characteristics of a bili-protein complex in the serum of the stargazer fish Genyagnus novazelandiae: Uranoscopidae) and its possible role in camouflage

Identification of the blue-green chromophore of an abundant biliprotein from the haemolymph of Artemia

Comparison of pigments in the integument of cobalt, albino, and normal rainbow trout, Salmo gairdnerii irideus

The weaker sex: Male lingcod (Ophiodon elongatus) with blue color polymorphism are more burdened by parasites than are other sex–color combinations