Activities and some properties of 5′nucleotidase in isolated hamster fat cells

doi:10.1016/0305-0491(85)90021-5

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry

Volume 82, Issue 3, 1985, Pages 549-553

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Abstract

1.
1. Kinetic parameters and the possible cellular location of 5′nucleotidase were studied in isolated hamster fat cells.
2.
2. Measurements of the enzyme were performed using 5′IMP instead of 5′AMP as substrate.
3.
3. The apparent V_max and K_m values obtained support the view that two forms of 5′nucleotidase, having different affinities, are present in hamster fat cells. The major form was located in the outer surface of plasma membrane and the other one inside the cell.
4.
4. The effects of ADP, adenosine and inosine on the enzyme were studied. ADP and adenosine were competitive inhibitors. The enzyme was not modified by inosine.

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Activities and some properties of 5′nucleotidase in isolated hamster fat cells

Abstract

Biochim. biophys. Acta

Metabolism

FEBS Lett.

Biochim. biophys. Acta

J. biol. Chem.

J. biol. Chem.

Archs Biochem. Biophys.

Biochim. biophys. Acta

Biochim. biophys. Acta

Biochim. biophys. Acta

Biochim. biophys. Acta

Biochim. biophys. Acta

J. Molec. Cell. Cardiol.

Biochim. biophys. Acta

The control of the metabolism and the hormonal role of adenosine

Essays in Biochemistry

The influence of adenosine and prostaglandins on regulating processes of cardiac flow: investigations in vitro and in vivo

Acta biol. med. germ.

A study of the conditions and mechanism of diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid

Biochem. J.