Derivatives of sperm-activating peptide III (SAP-III) associated with eggs of sand dollars, Clypeasterjaponicus and Astriclypeusmanni

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Abstract

  • 1.

    1. NIne sperm-activating peptides were isolated from the egg jelly of sand dollars Clypeaster japonicus and Astriclypeus manni, and their amino acid sequences were determined.

  • 2.

    2. The peptides elevated cyclic GMP and cyclic AMP concentrations in spermatozoa of respective species and caused a shift in the apparent molecular weight of a major sperm protein of the respective species of sand dollars.

  • 3.

    3. The peptides stimulated their own sperm respiration rate one half-maximally at about 0.1–1.9 nM under slightly acidic pH.

  • 4.

    4. Egg jelly extract from Peronella japonica contained a substance(s) which stimulates C. japonicus sperm respiration.

References (38)

  • N. Suzuki et al.

    A novel group of sperm activating peptides from the sea urchins Glyptocidaris crenularis

    Comp. Biochem. Physiol.

    (1988)
  • T. Takao et al.

    Verification of protein sequence by fast bombardment mass spectrometry

  • K. Yoshino et al.

    A halogenated amino acid-containing sperm-activating peptides and its related peptides isolated from the egg jelly of sea urchins, Tripneustes gratilla, Pseudoboletia maculata, Strongylocentrotus nudus, Echinometra mathaei and Heterocentrotus mammillatus

    Comp. Biochem. Physiol.

    (1989)
  • K. Yoshino et al.

    A species-specific sperm-activating peptide from the egg jelly of the sea urchin Diadema setosum

    Comp. Biochem. Physiol.

    (1990)
  • J.K. Bentley et al.

    Receptor-mediated activation of spermatozoan guanylate cyclase

    J. biol. Chem.

    (1986)
  • M.P. Bradley et al.

    The modulation of sperm metabolism and motility by factors associated with eggs

    Ann. N.Y. Acad. Sci.

    (1984)
  • D.L. Garbers

    Molecular basis of fertilization

    Ann. Rev. Biochem.

    (1989)
  • U.K. Laemmli

    Cleavage of structural proteins during the assembly of the head of bacteriophage T4

    Nature

    (1970)
  • H. Shimomura et al.

    Covalent coupling of a resact analogue to guanylate cyclase

    J. biol. Chem.

    (1986)
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