General paper
The fate of mevalonate in the crab: The case for CO2 and t-RNA containing isopentenyl-adenosine

https://doi.org/10.1016/0305-0491(86)90269-5Get rights and content
Under a Creative Commons license
open archive

Abstract

  • 1.

    1. The Maryland Blue Crab (Callinectes sapidus Rathbun) does not synthesize cholesterol; sterol ingestion yields its cholesterol.

  • 2.

    2. This crab was selected to study the fate of mevalonate, a required intermediary in cholesterol biosynthesis.

  • 3.

    3. Mevalonate yielded 82–94% of its label into CO2; but, <1% into isopentenyl-adenosine t-RNA (ia-t-RNA) and only a trace into dolichols.

  • 4.

    4. Identical labeling patterns to those used in mevalonate were found in ia-t-RNA; isopentenyl alcohol was recovered labeled; these facts suggest that mevalonate was utilized intact.

  • 5.

    5. The above results and the recovery of hydroxyl-methylglutarate labeled, suggests an active non-steroidal pathway for mevalonate.

Cited by (0)