A novel process of cyclodextrin production by the use of specific adsorbents: Part II. A new reactor system for selective production of α-cyclodextrin with specific adsorbent

https://doi.org/10.1016/0922-338X(91)90253-DGet rights and content

Abstract

We have developed a novel process of α-cyclodextrin (α-CD) production by using a new adsorbent that is characterized by its exceedingly powerful selectivity for α-CD compared with other CDs. α-CD production was carried out in a closed reactor system that was composed of a main reactor, wherein liquefied starch was converted to CDs by cyclodextrin glucosyltransferase (CGTase: EC 2.4.1.19), and a column packed with the adsorbent. While the reaction mixture was circulated in the system, α-CD was selectively adsorbed in the column and its concentration in the mixture of the main reactor was kept at a low level. This low concentration of α-CD stimulated the conversion of starch to CDs and as a result, enhanced its yield based on added starch. When 8.3 % (w/v) of liquefied starch was used in the reactor system, the yield of α-CD was 22.2% and α-CD occupied 58.7 % of the reaction mixture of total CDs synthesized. Meanwhile, in a batch system without the adsorbent, the yield of α-CD and its fraction were 10.8% and 45.0%, respectively. After the conversion reaction, and following the preliminary washing with water through the column. α-CD was easily eluted with hot water, resulting in a high purity of about 95%.

References (10)

  • N. Nelson

    A photometric adsorption of the Somogyi method for the determination of glucose

    J. Biol. Chem.

    (1944)
  • H. Hashimoto et al.

    Effective conditions on cyclodextrins preparation

    J. Jpn. Soc. Starch Sci.

    (1985)
  • S. Kobayashi

    Action mechanisms of B. macerans enzyme and preparation of cyclodextrins

    J. Jpn. Soc. Starch Sci.

    (1975)
  • F. Shiraishi et al.

    Effect of ethanol on formation of cyclodextrin from starch by Bacillus macerans cyclodextrin glucanotransferase

    Starch/Stärke

    (1989)
  • H. Hashimoto et al.

    Concentration of the conversion mixture solution by using reverse osmosis membrane

    J. Jpn. Soc. Starch Sci.

    (1985)
There are more references available in the full text version of this article.

Cited by (0)

View full text