The nucleotide sequence of the mouse embryonic β-like y-globin messenger RNA as determined from cloned cDNA

doi:10.1016/0378-1119(81)90070-6

Gene

Volume 16, Issues 1–3, December 1981, Pages 141-148

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Abstract

We have determined the nucleotide sequence of two cloned cDNAs corresponding to the mRNA of mouse embryonic y₂ globin. The combined overlapping sequences span a total of 480 bp, beginning at the codon corresponding to amino acid residue 21 and extending to the AATAAA sequence in the 3' untranslated region. Therefore, when the amino acid sequence encoded by the cDNA is combined with the available amino acid sequence, a complete y₂ protein sequence can be obtained.

Comparisons, at the nucleotide level, between the known β- and β-like globin sequences and the y₂ sequence show that the embryonic, fetal-adult duplication occurred approx. 160 million years (MY) ago and that the embryonic-fetal duplication occurred approx. 100 MY ago.

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Molecular cloning and sequence analysis of a cDNA coding for the mouse alpha-like embryonic globin chain x
1984, Gene
Cytoplasmic poly(A)⁺mRNA from 12-day mouse-yolk-sac erythroid cells has been used to prepare a cDNA library in the plasmid pBR322. One clone containing sequences coding for the α-like embryonic globin chain x, pHE52, has been identified by hybrid selection and in vitro translation of the complementary mRNA. The nucleotide sequence of pHE52 confirms that it codes for an embryonic α-like globin chain. The insert sequence is 316 nucleotides long, contains the codons corresponding to amino acid residues 43–141, and extends into the 3' untranslated region. An analysis of the nucleotide sequence of pHE52 and the other known α globins suggests that the adult-embryonic divergence began approx. 400 million years ago reflecting a difference in the evolutionary history of the α- and β-globin gene complexes.
Primitive erythropoiesis of mouse teratocarcinoma stem cells PCC3/A/1 in serum-free medium
1991, Development

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The nucleotide sequence of the mouse embryonic β-like y-globin messenger RNA as determined from cloned cDNA

Abstract

Cell

Gene

Cell

J. Mol. Biol.

Biochim. Biophys. Acta

Blood

Cell

Cell

Cell

Cell

Cell

J. Mol. Biol.

Cell

Cloning of a new mouse foetal β-globin mRNA sequence

Nucl. Acids Res.

Complete nucleotide sequence of the haemagglutinin gene from a human influenza virus of the Hong Kong subtype

Nucl. Acids Res.

Nucleotide sequences from the rabbit beta globin gene inserted into Escherichia coli plasmids

Science