Determination of tritiated digoxin and metabolites in urine by liquid chromatography

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Abstract

A liquid chromatographic method for the determination of digoxin, digoxigenin, its mono- and bisdigitoxoside and dihydrodigoxin in urine is described. Doses of 100 μCi of [12α-3H] digoxin and 0.5 mg (640 nmol) of digoxin were administered orally to eight healthy volunteers. The compounds were extracted from urine with methylene chloride containing 3% of heptafluorobutanol. After separation, fractions corresponding to digoxin and the metabolites were measured by liquid scintillation counting. Conjugates of the glycoside metabolites were determined indirectly after pre-treatment of the samples with β-glucuronidase—arylsulphatase. The detection limit was 0.1 nmol/l. Metabolites amounting to 0.5% of digoxin were assayed with a relative standard deviation of 5%.

The advantages of the method are a high recovery in the extraction step, short separation times and the possibility of separate assay of dihydrodigoxin.

References (19)

  • H. Greenwood et al.

    Clin. Chim. Acta

    (1975)
  • R.J. Luchi et al.

    Amer. J. Med.

    (1968)
  • E. Watson et al.

    J. Chromatogr.

    (1972)
  • M.C. Castle

    J. Chromatogr.

    (1975)
  • W. Lindner et al.

    J. Chromatogr.

    (1976)
  • H.A. Nelson et al.

    J. Chromatogr.

    (1979)
  • M.H. Gault et al.

    J. Chromatogr.

    (1980)
  • J. Kuhlmann et al.

    Naunyn-Schmiedebergs Arch. Pharmakol. Exp. Pathol.

    (1973)
  • M.H. Gault et al.

    J. Pharm. Pharmacol.

    (1977)
There are more references available in the full text version of this article.

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