Simultaneous determination of chlorpromazine and levomepromazine in human plasma and urine by high-performance liquid chromatography using electrochemical detection

https://doi.org/10.1016/S0378-4347(00)80360-6Get rights and content

Abstract

A rapid, selective and sensitive method for the simultaneous determination of chlorpromazine and levomepromazine in human plasma and urine has been developed using high-performance liquid chromatography with electrochemical detection.

The unchanged drugs and internal standard extracted from plasma and urine were separated by reversed-phase high-performance liquid chromatography. The influence of acetonitrile concentration and of the pH of the mobile phase were investigated. The detection limits were 100 pg for chlorpromazine and for levomepromazine. In comparison with three other detection systems this was found to be the most sensitive method.

This method was successfully applied to the simultaneous determination of chlorpromazine and levomepromazine in human plasma and urine for pharmacokinetic studies.

References (16)

  • G.W. Christoph et al.

    Clin. Chim. Acta

    (1972)
  • J.S. Noonan et al.

    Life Sci.

    (1972)
  • J.H. Knox et al.

    J. Chromatogr.

    (1975)
  • W. Lindner et al.

    J. Chromatogr.

    (1975)
  • J. Fekete et al.

    J. Chromatogr.

    (1981)
  • U.R. Tjaden et al.

    J. Chromatogr.

    (1976)
  • S. Merlis et al.
  • S.H. Curry

    Anal. Chem.

    (1968)
There are more references available in the full text version of this article.

Cited by (31)

  • Graphene paste electrode for detection of chlorpromazine

    2011, Electrochemistry Communications
    Citation Excerpt :

    The CPZ is a phenothiazine drug with an aliphatic side chain, used in the management of psychotic conditions [24]. Many methods have been reported for the quantitative determination of CPZ, including those involving voltammetric methods [25–27], HPLC [28–30] and gas chromatography [31,32]. However, most of these methods require several time consuming manipulation steps, sophisticated instruments and special training.

  • Determination of selected phenothiazines in human plasma by solid-phase extraction and liquid chromatography with coulometric detection

    2008, Analytica Chimica Acta
    Citation Excerpt :

    The selectivity and sensitivity of the analytical method are of paramount importance for the reliable TDM of patients due to the very low therapeutic plasma levels of some drugs (i.e. fluphenazine depot). Several papers can be found in the literature regarding the determination of some phenothiazines as a single analyte or together with other drugs in biological fluids or tissues [9–25]. Usually, the drugs are analysed by means of high-performance liquid chromatography (HPLC) coupled with UV detection [9,11,24,25], fluorescence detection [13], mass spectrometry [13] or electrochemical (both amperometric and coulometric) detection [18–23].

  • Simple and simultaneous determination for 12 phenothiazines in human serum by reversed-phase high-performance liquid chromatography

    2007, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
View all citing articles on Scopus
View full text