Microdetermination of 2,3-dinor-6-ketoprostaglandin F in human urine using gas chromatography—high-resolution selected-ion monitoring

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Abstract

The microdetermination of 2,3-dinor-6-ketoprostaglandin F (I) in human urine is described. Samples to which the [2H4]-analogue was added as an internal standard were extracted by chromatographic sample preparation using a Bond Elut C18 cartridge and a silica gel column. Conversion of the extracted I into the 1-methyl ester-6-methoxime-9,11,15-trisdimethylisopropylsilyl ether derivative was followed by gas chromatography—high-resolution selected-ion monitoring (GC—HR-SIM). Interfering substances from the urine matrix were eliminated during GC—HR-SIM analysis using a DB-1 column. A good linear response over the range 10 pg–100 ng per tube was demonstrated. Compound I could be detected in the range 26–375 pg/ml of human urine. The proposed method can be applied to the determination of I in human urine.

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