General paper
Cyclic AMP: The second messenger of an antidiuretic hormone from the glandular lobes of the migratory locust corpora cardiaca

https://doi.org/10.1016/0022-1910(87)90113-2Get rights and content

Abstract

Cyclic AMP levels in the rectum of the migratory locust were measured by means of a radioimmunoassay. An antidiuretic factor, extracted from the glandular lobes of the corpora cardiaca, enhances water reabsorption across the rectal wall in vitro (maximal response with 0.5 gland10 μl of bathing medium). The same extract also elicits an increase in the cyclic AMP of the rectal tissue (60% with 0.05 gland10 μl of bathing medium). Two analogues of cyclic AMP (2′-O-dibutyryl-cyclic AMP 1 mM and 8-Br-cyclic AMP 1 mM) enter the rectal cells and mimic this antidiuretic effect. Forskolin (0.1 mM) stimulates an increase of the cyclic AMP concentration at the rectal level by activating the adenylate cyclase. IBMX (0.2 mM), by inhibiting phosphodiesterase activity, dramatically potentiates the forskolin effect. Furthermore, forskolin mimics the action of the antidiuretic factor. These results lead us to conclude that cyclic AMP is a likely second messenger of the antidiuretic factor contained in the glandular lobes of the corpora cardiaca.

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