Effect of adding fibre and starch degrading enzymes to a barley/wheat based diet on performance and nutrient digestibility in different segments of the small intestine of early weaned pigs
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Effect of xylanase and β-glucanase on growth performance, activity of digestive enzymes, digestibility, and microbiome diversity of juvenile Nile tilapia fed soybean meal and/or sorghum distillers dried grains with solubles-based diets
2023, AquacultureCitation Excerpt :Then, 1e mL of enzyme dilution was incubated with 1 mL of substrate solution at 50 °C for 60 min. The reducing sugars were assayed by adding 3 mL of 3, 5-dinitrosalicylic acid reagent, boiling for 5 min, and the absorbance was measured at 540 nm (Inborr et al., 1993). Additionally, dietary β-glucanase (endo-1,4-β-glucanase) activity was performed by adding 1 mL of PC buffer into 0.75 g of barley.
Xylanase and β-glucanase improve growth performance, gut barrier, and microbiota of pre-growout Nile tilapia, Oreochromis niloticus fed a vegetable-based diet
2022, AquacultureCitation Excerpt :One ml of enzyme dilution was incubated with 1 mL of substrate solution at 50 °C for 60 min. Reducing sugars were assayed by adding 3 mL of 3, 5-dinitrosalicylic acid reagent, boiling for 5 min, and measuring the absorbance at 540 nm (Inborr et al., 1993). Dietary β-glucanase (endo-1, 4)-glucanase) activity was determined by adding 1 mL of PC buffer into 0.75 g of barley.
Perspectives of nonstarch polysaccharide enzymes in nutrition
2018, Enzymes in Human and Animal Nutrition: Principles and PerspectivesThe effect of adding xylanase or β-glucanase to diets with corn distillers dried grains with solubles (CDDGS) on growth performance and nutrient digestibility in nursery pigs
2017, Livestock ScienceCitation Excerpt :However, other studies reported no benefit of xylanase or Beta-glucanase addition (Officer, 1995; Olukosi et al., 2007; Thacker et al., 1992). The same type of enzymes with different strains and preparation methods can result in different result (Inborr et al., 1993; Vahjen and Simon, 1999). The XYL, BGL, and XB used in this study showed ability to work on fiber from CDDGS, however, further studies are required to investigate why the improved digestibility did not translate into improve nutrient utilization.
Characterization of dual enzyme resulted from bicistronic expression of two β-glucanases in porcine cells
2015, Journal of Integrative Agriculture