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Plant Protein Agar, a Medium for the Isolation and Estimation of Proteolytic Organisms in Grassland Soils

Abstract

GELATIN, casein and occasionally albumen are used for isolation and counting of proteolytic soil organisms. Among the principal substrates for such organisms in grassland soils will be the dead tillers, the sloughed off roots and, after ploughing, the stubble and root mat of the sward. Since plant protein will be present in plant residues, its choice as a medium for proteolytic soil organisms seems in this case more logical than that of either gelatin, casein or albumen, proteins of animal origin. Waksman and Starkey1 have examined the decomposition of vegetable proteins by some soil organisms.

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References

  1. Waksman, S. A., and Starkey, R. J., J. Bact., 33, 405 (1932).

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  2. Grossbard, Erna, and Hall, D. M., in Grassland Res. Inst. Experiments in Progress, No. 12, 85 (1959).

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  3. Grossbard, Erna, and Hall, D. M., in Grassland Res. Inst. Experiments in Progress, No. 14 (in the press).

  4. Grossbard, Erna, and Hall, D. M., in Grassland Res. Inst. Experiments in Progress, No. 13, 70 (1960).

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  5. Skinner, F. A., J. Gen. Microbiol., 22, 539 (1960).

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GROSSBARD, E., HALL, D. Plant Protein Agar, a Medium for the Isolation and Estimation of Proteolytic Organisms in Grassland Soils. Nature 196, 1119–1120 (1962). https://doi.org/10.1038/1961119a0

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  • DOI: https://doi.org/10.1038/1961119a0

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